Resveratrol Supplement Could Reverse the Effect of Resistance Training on Muscular eMHC and PAX7 in BALB/C Mice Bearing CT-26 Tumor

Document Type : Original Article


Department of Physical Education and Sport Sciences, Shiraz University, Shiraz, Iran


Introduction: Although resistance training and resveratrol is recommended as a stimulant for muscle regeneration in healthy subjects their effects on cancer-related cachexia indices are unclear. This study aimed to investigate the effect of a course of resistance training with resveratrol supplement on PAX-7 and eMHC in Gastrocnemius muscle tissue of mice bearing colon cancer induced cachexia. Methods: This was an experimental study conducted on 20 six-week-old BALB/c mice implanted with CT-26 tumor. The mice were randomly divided into four groups: resistance training, resveratrol, resveratrol with resistance training, and control. The resistance training group performed 6 weeks of progressive resistance training, the resveratrol group received 100 mg/kg resveratrol per day. 48 hours after the last experimental session, the mice were sacrificed and PAX-7 and eMHC protein levels were measured using ELISA test through specimen taken from gastrocnemius muscles. Statistical test of one way ANOVA was used for data analysis. Results: PAX-7 level was significantly lower in resveratrol with resistance training group compared to control (P=0.009), resveratrol (P=0.005), and resistance training groups (P=0.002). eMHC was significantly lower in the resistance training group compared to the control (P=0.08), resistance training, and resveratrol groups (P=0.002). Conclusion: Resistance training was not an appropriate intervention for treating reduced muscular regeneration due to cachexia. However, regarding reducing effect of resveratrol supplementation with resistance training on PAX-7 level, it may be recommended to improve muscular regeneration.


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