The Effects of Centella asiatica Aqueous Extract on some Blood Parameters in Rat Model of Alzheimer's Disease

Document Type : Original Article


1 Cellular and Molecular Biology Department, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran

2 Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran


Introduction: Alzheimer’s disease (AD) is a progressive and irreversible neuropsychiatric disorder of the brain with a deadly outcome and unknown etiology in which the concentration and function of some brain transmitters, particularly acetylcholine, reduce in AD in the cerebral cortex, e.g., the hippocampus. This study aimed to evaluate the effects of aquatic extract of Centella asiatica (CeA) leaf on hematological parameters in NBM (Nucleus Basalis of Mynert)- lesioned rats induced by Ibotenic acid (IA). Methods: Thirty-two male Wistar rats (20- 24 months old; weighing 330±30 g) were selected and randomly divided into four equal groups (n=12) including 1. Control group (which were intact rats), 2. L group (NBM- lesioned rats), 3. CeA group and 4. L+CeA group. NBM was bilaterally lesioned. Seven days after stereotaxic surgery, the total number of white blood cells (WBCs), the total number of red blood cells (RBCs), hemoglobin level (Hb), and hematocrit level (Hct) were assessed. For statistical analysis of data Mann Whitney U- test (p≤0.05) was used. Results: IA treatment induced a significant decrease of WBCs, followed by significant decrease in RBCs, Hb and Hct compared with control group (p≤0.05). Also, the lesion of the NBM after oral administration of Centella asiatica extract had no effect on the above blood parameters and even all of the parameters in two groups of CeA and CeA+L compared with the control group showed a non-significant increase (p≥0.05). Conclusion: It appears that some blood parameters decrease in rat model of Alzheimer's disease. Also, this research shows the important role of the cholinergic neurons of central nervous system in the regulation of erythropoiesis, also Centella asiatica extract can stimulate hematopoiesis.


1.Scuderi C, Stecca C, Bronzuoli MR, Rotili D, Valente S, Mai A, et al. Sirtuin modulators control reactive gliosis in an in vitro model of Alzheimer's disease. Front Pharmacol. 2014; 5: 1- 8.
2.Gray NE, Morre J, Kelley J, Maier CS, Stevens JF, Quinn JF, et al. Caffeoylquinic acids in Centella asiatica protect against amyloid-beta toxicity. J Alzheimers Dis. 2014; 40: 359- 373.
3.Collingridge GL, Singer W. Excitatory amino acid receptors and synaptic plasticity. Trends Pharmacol Sci. 1990; 11: 290- 296.
4.Chong ZZ, Li F, Maiese K. Stress in the brain: novel cellular mechanisms of injury linked to Alzheimer's disease. Brain Res Brain Res Rev. 2005; 49: 1- 21.
5.Arendt T. Synaptic plasticity and cell cycle activation in neurons are alternative effector pathways: the 'Dr. Jekyll and Mr. Hyde concept' of Alzheimer's disease or the yin and yang of neuroplasticity. Prog Neurobiol. 2003; 71: 83- 248.
6.Garcia-Alloza M, Hirst WD, Chen CP, Lasheras B, Francis PT, Ramirez MJ. Differential involvement of 5-HT(1B/1D) and 5-HT6 receptors in cognitive and non-cognitive symptoms in Alzheimer's disease. Neuropsychoph. 2004; 29: 410- 416.
7.Wan B HX, Nie J, Zhou M, Yang B, Li Y, Wen W, Lü C. Effects of triptolide on degeneration of dendritic spines induced by Aβ1-40 injection in rat hippocampus. Neurol Sci. 2014; 35: 35- 40.
8.Miranda M, Ferreira G, Ramı, X, et al. Role of cholinergic system on the construction of memories: Taste memory encoding. Neurobiol Learn Mem. 2003; 80: 211- 222.
9.Etienne P, Robitaille Y, Wood P, Gauthier S, Nair NP, Quirion R. Nucleus basalis neuronal loss, neuritic plaques and choline acetyltransferase activity in advanced Alzheimer's disease. J Neurosci. 1986; 19: 1279- 1291.
10.Soumyanath A, Zhong Y-P, Henson E, Wadsworth T, Bishop J, Gold BG, et al. Centella asiatica extract improves behavioral deficits in a mouse model of alzheimer's disease: investigation of a possible mechanism of action. Int J Alzheimers Dis. 2012; 1: 1- 9.
11.Huang MX, Liu WJ. Effect of cluster a in Hox gene on proliferation and differentiation of hematopoietic stem/progenitor cells and its relation to leukemia -- review. Zhongguo Shi. 2009; 17: 835- 839.
12.Desforges JF. Regulation of hematopoiesis: 14th annual paul aggeler memorial lecture. Wes J Med. 1984; 141: 88- 92.
13.Khaldoyanidi S, Sikora L, Orlovskaya I, Matrosova V, Kozlov V, Sriramarao P. Correlation between nicotine-induced inhibition of hematopoiesis and decreased CD44 expression on bone marrow stromal cells. Blood J. 2001; 98: 303- 312.
14.Serobyan N, Orlovskaya I, Kozlov V, Khaldoyanidi SK. Exposure to nicotine during gestation interferes with the colonization of fetal bone marrow by hematopoietic stem/progenitor cells. Stem Cells Dev. 2005; 14: 81- 91.
15.Heidari M, Heidari-Vala H, Sadeghi MR, Akhondi MM. The inductive effects of Centella asiatica on rat spermatogenic cell apoptosis in vivo. J Nat Med. 2012; 66: 271- 278.
16.Wang QH, Xu RX, Nagao S. Transplantation of cholinergic neural stem cells in a mouse model of Alzheimer's disease. Chin Med J. 2005; 118: 508- 511.
17.Doulah AH, Rohani AH, Khaksari Haddad M, Motamedi F, Farbood Y, Badavi M, et al. The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned rats. Neurosci Lett. 2009; 466: 47- 51.
18.BÜTtner-Ennever J. The rat brain in stereotaxic coordinates, 3rd edn. J Anat. 1997; 191: 315- 317.
19.Keikhaei B, Rahim F, Zandian K, Pedram M. Comparison of different indices for better differential diagnosis of iron deficiency anemia from beta thalassemia trait. Sci J Iran Blood Transfus Organ. 2007; 4: 95- 104.
20.Mlekusch W, Truppe W, Beyer W, Paletta B. The effect of hunger on free fatty acid and corticosterone plasma levels in rats. Experientia. 1975; 31: 1135- 1137.
21.Pang LL, Hou LB, Mei QX, Kong XL, Hu Y, Gao YQ, et al. Effects of compound Centella asiatica enema on kidneys coefficient, electrolytes and blood in chronic renal failure rats. Zhong Yao Cai. 2010; 33: 775- 778.
22.Rahim F, Keikhaei B, Sarkaki A, Hassan Doulah A. Ibotenic acid-induced hematological disorders in rat model of alzheimer's disease. Asian J Anim Vet Adv. 2010; 5: 13- 23.
23.Maneewan C, Mekbungwan A, Charerntantanakul W, Yamauchi K, Yamauchi KE. Effects of dietary Centella asiatica (L.) Urban on growth performance, nutrient digestibility, blood composition in piglets vaccinated with mycoplasma hyopneumoniae. Anim Sci J. 2014; 85: 569- 574.